Sex-related differences in crossing over in Caenorhabditis elegans.
نویسندگان
چکیده
In the nematode Caenorhabditis elegans, hermaphrodite recombination has been characterized and is the basis of the genetic map used in this organism. In this study we have examined male recombination on linkage group I and have found it to be approximately one-third less than that observed in the hermaphrodite. This decrease was interval-dependent and nonuniform. We observed less recombination in the male in 5 out of 6 intervals examined, and no observable difference in one interval on the right end of LG I. Hermaphrodite recombination frequencies are the result of recombination in two germlines; oocyte and hermaphrodite spermatocytes. We have measured recombination in the oocyte and have found it to be approximately twofold lower than that calculated for hermaphrodite spermatocytes and not significantly different from the male spermatocyte frequency. Thus, recombination frequencies appear to be a function of gonad physiology rather than the sex of the germline. Evidence from experiments examining the effect of karyotype on recombination in males sexually transformed by the her-1 mutation into XO hermaphrodites (normally XX), suggests the sexual phenotype rather than genotype determines the recombination frequency characteristic of a particular sex. Hermaphrodite recombination is known to be affected by temperature, maternal age, and the rec-1 mutation. We have examined the effect of these parameters on recombination in the male and have found male recombination frequency increased with elevated temperatures and in the presence of Rec-1, and decreased with paternal age.
منابع مشابه
SNP-based mapping of crossover recombination in Caenorhabditis elegans.
Caenorhabditis elegans is an important experimental organism for the study of recombination during meiosis. Here, we provide methods for the use of single-nucleotide polymorphisms (SNPs) for the study of crossing over in C. elegans.
متن کاملDistributed effects of biological sex define sex-typical motor behavior in Caenorhabditis elegans.
Sex differences in shared behaviors (for example, locomotion and feeding) are a nearly universal feature of animal biology. Though these behaviors may share underlying neural programs, their kinematics can exhibit robust differences between males and females. The neural underpinnings of these differences are poorly understood because of the often-untested assumption that they are determined by ...
متن کاملTocotrienol Modulates the Expression of Proteins in Oxidative Stress-Induced Caenorhabditis Elegans
Objective: Oxidative stress that damages proteins result in aging and age related diseases. The aim of this study is to determine the effect of tocotrienol rich fraction (TRF) on the expression of proteins in oxidative stress-induced caenohabditis elegans (C.elegans) which has homologous genes to humans. Methods: The worms were treated with TRF prior to, after and continuously in separate group...
متن کاملاستفاده از تعامل نماتد Caenorhabditis elegans، قارچ Arthrobotrys oligospora و باکتری Bacillus subtilis در کنترل نماتد Meloidogyne javanica
در این تحقیق از تعامل نماتد Caenorhabditis elegans، قارچ Arthrobotrys oligospora و باکتری Bacillus subtilis در کنترل نماتد مولد گره ریشه Meloidogyne javanica استفاده شد. باکتری مذکور جهت تحریک سیستم دفاعی گیاه در بدو تیمار و به عنوان غذای نماتد C. elegans و نماتد C. elegans به منظور افزایش تولید تله استفاده شد. قارچoligospora A. پس از 72 ساعتموجب مرگ و میر 77% لاروهای نماتد M. javanica گردید، ...
متن کاملPolyploids and sex determination in Caenorhabditis elegans.
Tetraploid stocks of Caenorhabditis elegans var. Bristol carrying autosomal and X-linked markers have been produced. Tetraploid hermaphrodites fall into two categories: those that give about 1% male self-progeny and those that give 25 to 40% male self-progeny. The former are basically 4A;4X--four sets of autosomes and four sex chromosomes--and the latter are 4A;3X. Males are 4A;2X. (Diploid her...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Genetics
دوره 126 2 شماره
صفحات -
تاریخ انتشار 1990